An in silico proteomics screen to predict and prioritize protein-protein interactions dependent on post-translationally modified motifs.

ABSTRACT:

Motivation

The development of proteomic methods for the characterization of domain/motif interactions has greatly expanded our understanding of signal transduction. However, proteomics-based binding screens have limitations including that the queried tissue or cell type may not harbor all potential interacting partners or post-translational modifications (PTMs) required for the interaction. Therefore, we sought a generalizable, complementary in silico approach to identify potentially novel motif and PTM-dependent binding partners of high priority.

Results

We used as an initial example the interaction between the Src homology 2 (SH2) domains of the adaptor proteins CT10 regulator of kinase (CRK) and CRK-like (CRKL) and phosphorylated-YXXP motifs. Employing well-curated, publi

SUBMITTER: Schmoker AM 

PROVIDER: S-EPMC6223376 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature