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Immune activation and regulatory T cells in Mycobacterium tuberculosis infected lymph nodes.


ABSTRACT:

Background

Lymph node tuberculosis (LNTB) is the most frequent extrapulmonary form of tuberculosis (TB). Studies of human tuberculosis at sites of disease are limited. LNTB provides a unique opportunity to compare local in situ and peripheral blood immune response in active Mycobacterium tuberculosis (Mtb) disease. The present study analysed T regulatory cells (Treg) frequency and activation along with CD4+ T cell function in lymph nodes from LNTB patients.

Results

Lymph node mononuclear cells (LNMC) were compared to autologous peripheral blood mononuclear cells (PBMC). LNMC were enriched for CD4+ T cells with a late differentiated effector memory phenotype. No differences were noted in the frequency and mutifunctional profile of memory CD4+ T cells specific for Mtb. The proportion of activated CD4+ and Tregs in LNMC was increased compared to PBMC. The correlation between Tregs and activated CD4+ T cells was stronger in LNMC than PBMC. Tregs in LNMC showed a strong positive correlation with Th1 cytokine production (IL2, IFN? and TNF?) as well as MIP-1? after Mtb antigen stimulation. A subset of Tregs in LNMC co-expressed HLA-DR and CD38, markers of activation.

Conclusion

Further research will determine the functional relationship between Treg and activated CD4+ T cells at lymph node sites of Mtb infection.

SUBMITTER: Sahmoudi K 

PROVIDER: S-EPMC6225640 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Publications

Immune activation and regulatory T cells in Mycobacterium tuberculosis infected lymph nodes.

Sahmoudi Karima K   Abbassi Hassan H   Bouklata Nada N   El Alami Mohamed Nouredine MN   Sadak Abderrahmane A   Burant Christopher C   Henry Boom W W   El Aouad Rajae R   Canaday David H DH   Seghrouchni Fouad F  

BMC immunology 20181108 1


<h4>Background</h4>Lymph node tuberculosis (LNTB) is the most frequent extrapulmonary form of tuberculosis (TB). Studies of human tuberculosis at sites of disease are limited. LNTB provides a unique opportunity to compare local in situ and peripheral blood immune response in active Mycobacterium tuberculosis (Mtb) disease. The present study analysed T regulatory cells (Treg) frequency and activation along with CD4+ T cell function in lymph nodes from LNTB patients.<h4>Results</h4>Lymph node mono  ...[more]

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