Ontology highlight
ABSTRACT: Background
Lymph node tuberculosis (LNTB) is the most frequent extrapulmonary form of tuberculosis (TB). Studies of human tuberculosis at sites of disease are limited. LNTB provides a unique opportunity to compare local in situ and peripheral blood immune response in active Mycobacterium tuberculosis (Mtb) disease. The present study analysed T regulatory cells (Treg) frequency and activation along with CD4+ T cell function in lymph nodes from LNTB patients.Results
Lymph node mononuclear cells (LNMC) were compared to autologous peripheral blood mononuclear cells (PBMC). LNMC were enriched for CD4+ T cells with a late differentiated effector memory phenotype. No differences were noted in the frequency and mutifunctional profile of memory CD4+ T cells specific for Mtb. The proportion of activated CD4+ and Tregs in LNMC was increased compared to PBMC. The correlation between Tregs and activated CD4+ T cells was stronger in LNMC than PBMC. Tregs in LNMC showed a strong positive correlation with Th1 cytokine production (IL2, IFN? and TNF?) as well as MIP-1? after Mtb antigen stimulation. A subset of Tregs in LNMC co-expressed HLA-DR and CD38, markers of activation.Conclusion
Further research will determine the functional relationship between Treg and activated CD4+ T cells at lymph node sites of Mtb infection.
SUBMITTER: Sahmoudi K
PROVIDER: S-EPMC6225640 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Sahmoudi Karima K Abbassi Hassan H Bouklata Nada N El Alami Mohamed Nouredine MN Sadak Abderrahmane A Burant Christopher C Henry Boom W W El Aouad Rajae R Canaday David H DH Seghrouchni Fouad F
BMC immunology 20181108 1
<h4>Background</h4>Lymph node tuberculosis (LNTB) is the most frequent extrapulmonary form of tuberculosis (TB). Studies of human tuberculosis at sites of disease are limited. LNTB provides a unique opportunity to compare local in situ and peripheral blood immune response in active Mycobacterium tuberculosis (Mtb) disease. The present study analysed T regulatory cells (Treg) frequency and activation along with CD4+ T cell function in lymph nodes from LNTB patients.<h4>Results</h4>Lymph node mono ...[more]