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Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia.


ABSTRACT: NMR and chromatography methods combined with mass spectrometry are the most important analytical techniques employed for plant metabolomics screening. Metabolomic analysis integrated to transcriptome screening add an important extra dimension to the information flow from DNA to RNA to protein. The most useful NMR experiment in metabolomics analysis is the proton spectra due the high receptivity of 1H and important structural information, through proton-proton scalar coupling. Routinely, databases have been used in identification of primary metabolites, however, there is currently no comparable data for identification of secondary metabolites, mainly, due to signal overlap in normal 1H NMR spectra and natural variation of plant. Related to spectra overlap, alternatively, better resolution can be find using 1H pure shift and 2D NMR pulse sequence in complex samples due to spreading the resonances in a second dimension. Thus, in data brief we provide a catalogue of metabolites and expression levels of genes identified in soy leaves and roots under flooding stress.

SUBMITTER: Coutinho ID 

PROVIDER: S-EPMC6226558 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia.

Coutinho Isabel Duarte ID   Henning Liliane Marcia Mert LMM   Döpp Silas Aurelian SA   Nepomuceno Alexandre A   Moraes Larissa Alexandra Cardoso LAC   Marcolino-Gomes Juliana J   Richter Christian C   Schwalbe Harald H   Colnago Luiz Alberto LA  

Data in brief 20181015


NMR and chromatography methods combined with mass spectrometry are the most important analytical techniques employed for plant metabolomics screening. Metabolomic analysis integrated to transcriptome screening add an important extra dimension to the information flow from DNA to RNA to protein. The most useful NMR experiment in metabolomics analysis is the proton spectra due the high receptivity of <sup>1</sup>H and important structural information, through proton-proton scalar coupling. Routinel  ...[more]

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