Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos.

ABSTRACT: Selection of optimal primer pairs in 16S rRNA gene sequencing is a pivotal issue in microorganism diversity analysis. However, limited effort has been put into investigation of specific primer sets for analysis of the bacterial diversity of aging flue-cured tobaccos (AFTs), as well as prediction of the function of the bacterial community. In this study, the performance of four primer pairs in determining bacterial community structure based on 16S rRNA gene sequences in AFTs was assessed, and the functions of genes were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Results revealed that the primer set 799F-1193R covering the amplification region V5V6V7 gave a more accurate picture of the bacterial community structure of AFTs, wit

SUBMITTER: Wang F 

PROVIDER: S-EPMC6230335 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature