Dataset Information

Lignin-Degrading Abilities of Novel Autochthonous Fungal?Isolates Trametes hirsuta F13 and Stereum gausapatum F28.

ABSTRACT: The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T. hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84% with T. hirsuta F13 and 28.8% with S. gausapatum F28.

SUBMITTER: Jovic J

PROVIDER: S-EPMC6233014 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28.

Jović Jelena J Buntić Aneta A Radovanović Neda N Radovanović Neda N Petrović Bojan B Mojović Ljiljana L

Food technology and biotechnology 20180901 3

The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in va ...[more]

PMID: 30510479

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Project description:Fungi are organisms with the highest natural capacity to degrade lignocellulose substrates, which is enabled by complex systems of extracellular enzymes, whose expression and secretion depend on the characteristics of substrates and the environment.This study reports a secretome analysis for white-rot basidiomycete Trametes hirsuta cultivated on a synthetic media and a lignocellulose substrate. We demonstrate that T. hirsuta st. 072 produces multiple extracellular ligninolytic, cellulolytic, hemicellulolytic, peroxide generating, and proteolytic enzymes, as well as cerato-platanins. In contrast to other white rot species described earlier, which mostly secreted glucanases and mannosidases in response to the presence of the lignocellulose substrate, T. hirsuta expressed a spectrum of extracellular cellulolytic enzymes containing predominantly cellobiases and xylanases. As proteomic analysis could not detect lignin peroxidase (LiP) among the secreted lignin degrading enzymes, we attributed the observed extracellular LiP - like activity to the expressed versatile peroxidase (VP). An accessory enzyme, glyoxal oxidase, was found among the proteins secreted in the media during submerged cultivation of T. hirsuta both in the presence and in the absence of copper. However, aryl-alcohol oxidase (AAO) was not identified, despite the presence of AAO enzymatic activity secreted by the fungus. The spectra of the expressed enzymes dramatically changed depending on the growth conditions. Transfer from submerged cultivation to surface cultivation with the lignocellulose substrate switched off expression of exo-?-1,3-glucanase and ?-amylase and turned on secretion of endo-?-1,3-glucanase and a range of glycosidases. In addition, an aspartic peptidase started being expressed instead of family S53 protease. For the first time, we report production of cerato-platanin proteins by Trametes species. The secretion of cerato-platanins was observed only in response to contact with lignocellulose, thus indicating a specific role of these proteins in degradation of the lignocellulose substrates.Our results suggest a sequential mechanism of natural substrate degradation by T. hirsuta, in which the fungus produces different sets of enzymes to digest all main components of the substrate during cultivation.

Dataset Information

Lignin-Degrading Abilities of Novel Autochthonous Fungal?Isolates Trametes hirsuta F13 and Stereum gausapatum F28.

Publications

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates <i>Trametes hirsuta</i> F13 and <i>Stereum gausapatum</i> F28.

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Dataset Information

Lignin-Degrading Abilities of Novel Autochthonous Fungal?Isolates Trametes hirsuta F13 and Stereum gausapatum F28.

Publications

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates <i>Trametes hirsuta</i> F13 and <i>Stereum gausapatum</i> F28.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates <i>Trametes hirsuta</i> F13 and <i>Stereum gausapatum</i> F28.