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ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing.


ABSTRACT: Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson-Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in living Arabidopsis thaliana seedlings, we found that MgATP2- concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) in Arabidopsis mesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP:NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.

SUBMITTER: Voon CP 

PROVIDER: S-EPMC6233094 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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ATP compartmentation in plastids and cytosol of <i>Arabidopsis thaliana</i> revealed by fluorescent protein sensing.

Voon Chia Pao CP   Guan Xiaoqian X   Sun Yuzhe Y   Sahu Abira A   Chan May Ngor MN   Gardeström Per P   Wagner Stephan S   Fuchs Philippe P   Nietzel Thomas T   Versaw Wayne K WK   Schwarzländer Markus M   Lim Boon Leong BL  

Proceedings of the National Academy of Sciences of the United States of America 20181023 45


Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson-Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein express  ...[more]

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