Quantification of species-specific meat proteins in cooked and smoked sausages using infusion mass spectrometry.

ABSTRACT: Label-free quantification combined with high-resolution infusion-based mass spectrometry (MS) was evaluated to authenticate 'horse sausages' made from horse meat and pork. Four types of industrially processed sausages, including cooked horse meat, pork and beef, and their mixtures were analysed. Quantitation and evaluation of the species composition were based on a set of 11 species-specific meat proteins and 14 unique heat-stable peptide markers. Using infusion MS, the highest distinguishing value was found in four proteins, namely, horse myosin-7 (MYH7_HORSE) and horse myoglobin (MYG_HORSE), porcine myosin-4 (MYH4_PIG) and bovine myoglobin (MYG_BOVIN). The limit of detection was 5% (w/w) for pork and beef in the three-component matrix and 1% (w/w) for horse meat. The proteins' abundance

SUBMITTER: Montowska M 

PROVIDER: S-EPMC6233461 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature