Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production.

ABSTRACT:

Background

Classic metabolic engineering strategies often induce significant flux imbalances to microbial metabolism, causing undesirable outcomes such as suboptimal conversion of substrates to products. Several mathematical frameworks have been developed to understand the physiological and metabolic state of production strains and to identify genetic modification targets for improved bioproduct formation. In this work, a modeling approach was applied to describe the physiological behavior and the metabolic fluxes of a shikimic acid overproducing Escherichia coli strain lacking the major glucose transport system, grown on complex media.

Results

The obtained flux distributions indicate the presence of high fluxes through the pentose phosphate and Entner-Doudoroff pathways, wh

SUBMITTER: Martinez JA 

PROVIDER: S-EPMC6233605 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature