Ontology highlight
ABSTRACT:
SUBMITTER: Chen Z
PROVIDER: S-EPMC6234918 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Chen Zhenyu Z Ren Xudong X Xu Xiangjie X Zhang Xiaojie X Hui Yi Y Liu Zhongliang Z Shi Lei L Fang Yujiang Y Ma Lin L Liu Yang Y Terheyden-Keighley Daniel D Liu Ling L Zhang Xiaoqing X
Stem cell reports 20181025 5
It is highly desirable to specify human developmental principles in an appropriate human model with advanced genetic tools. However, genetically engineering human cells with lineage-tracing systems has not been achieved. Here we introduce strategies to construct lineage-tracing systems in human embryonic stem cells (hESCs). The AAVS1 locus was suitable for the integration of the conditional reporter. The Cre-LoxP and Flp-FRT systems were highly sensitive, which may cause inaccurate lineage label ...[more]