Dataset Information

Changes in chemical and ultrastructural composition of ameroid constrictors following in vitro expansion.

ABSTRACT:

Objective

To (1) characterise the chemical and ultra-structural composition of ameroid constrictors, at a native state and during in vitro expansion and (2) determine the presence of irritant compounds at the surface or within the bulk of the constrictor.

Methods

Twelve sterile, commercially packaged ameroid constrictors (3 repeats of 3.5 mm, 5 mm, 6 mm and 7 mm internal diameter) were analysed by time-of-flight secondary ion mass spectrometry, Raman spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy and scanning electron microscopy.

Results

Ameroid constrictors have a composition commensurate with casein with little-to-no intra- or inter- constrictor variation. Microscopic analysis indicated that the topographical features of the constrictor surfaces were consistent between all constrictors. Following in vitro expansion there was a reproducible decrease in Ca+ ion content, little-to-no variation in secondary protein structure and morphological changes including the presence of surface aggregates present only at the inner surface of the ameroid constrictor. The potential irritant polydimethylsiloxane was found on the constrictor surface. A trace quantity of an ion fragment assigned as formaldehyde was detected; however, the extremely low level is thought highly unlikely to play a role as an inflammatory trigger clinically.

Discussion

There is a high degree of inter- and intra-constrictor homogeneity from different batches, and reproducible ultrastructural changes following in vitro expansion. Variations occur in both the surface chemistry and topography of the device during closure, which can potentially affect the biomaterial-host interface. Ameroid constrictor closure mechanism is likely involving calcium-mediated inter-protein interactions rather than the imbibition of water only.

SUBMITTER: Anderson TS

PROVIDER: S-EPMC6237400 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Changes in chemical and ultrastructural composition of ameroid constrictors following in vitro expansion.

Anderson Thomas S TS Rance Graham A GA Jiang Long L Piggott Matthew J MJ Field Elinor J EJ Chanoit Guillaume P GP

PloS one 20181115 11

<h4>Objective</h4>To (1) characterise the chemical and ultra-structural composition of ameroid constrictors, at a native state and during in vitro expansion and (2) determine the presence of irritant compounds at the surface or within the bulk of the constrictor.<h4>Methods</h4>Twelve sterile, commercially packaged ameroid constrictors (3 repeats of 3.5 mm, 5 mm, 6 mm and 7 mm internal diameter) were analysed by time-of-flight secondary ion mass spectrometry, Raman spectroscopy, attenuated total ...[more]

PMID: 30440023

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Dataset Information

Changes in chemical and ultrastructural composition of ameroid constrictors following in vitro expansion.

Objective

Methods

Results

Discussion

Publications

Changes in chemical and ultrastructural composition of ameroid constrictors following in vitro expansion.

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