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Consensus guidelines for the use and interpretation of angiogenesis assays.

ABSTRACT: The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.

SUBMITTER: Nowak-Sliwinska P

PROVIDER: S-EPMC6237663 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Consensus guidelines for the use and interpretation of angiogenesis assays.

Nowak-Sliwinska Patrycja P Alitalo Kari K Allen Elizabeth E Anisimov Andrey A Aplin Alfred C AC Auerbach Robert R Augustin Hellmut G HG Bates David O DO van Beijnum Judy R JR Bender R Hugh F RHF Bergers Gabriele G Bikfalvi Andreas A Bischoff Joyce J Böck Barbara C BC Brooks Peter C PC Bussolino Federico F Cakir Bertan B Carmeliet Peter P Castranova Daniel D Cimpean Anca M AM Cleaver Ondine O Coukos George G Davis George E GE De Palma Michele M Dimberg Anna A Dings Ruud P M RPM Djonov Valentin V Dudley Andrew C AC Dufton Neil P NP Fendt Sarah-Maria SM Ferrara Napoleone N Fruttiger Marcus M Fukumura Dai D Ghesquière Bart B Gong Yan Y Griffin Robert J RJ Harris Adrian L AL Hughes Christopher C W CCW Hultgren Nan W NW Iruela-Arispe M Luisa ML Irving Melita M Jain Rakesh K RK Kalluri Raghu R Kalucka Joanna J Kerbel Robert S RS Kitajewski Jan J Klaassen Ingeborg I Kleinmann Hynda K HK Koolwijk Pieter P Kuczynski Elisabeth E Kwak Brenda R BR Marien Koen K Melero-Martin Juan M JM Munn Lance L LL Nicosia Roberto F RF Noel Agnes A Nurro Jussi J Olsson Anna-Karin AK Petrova Tatiana V TV Pietras Kristian K Pili Roberto R Pollard Jeffrey W JW Post Mark J MJ Quax Paul H A PHA Rabinovich Gabriel A GA Raica Marius M Randi Anna M AM Ribatti Domenico D Ruegg Curzio C Schlingemann Reinier O RO Schulte-Merker Stefan S Smith Lois E H LEH Song Jonathan W JW Stacker Steven A SA Stalin Jimmy J Stratman Amber N AN Van de Velde Maureen M van Hinsbergh Victor W M VWM Vermeulen Peter B PB Waltenberger Johannes J Weinstein Brant M BM Xin Hong H Yetkin-Arik Bahar B Yla-Herttuala Seppo S Yoder Mervin C MC Griffioen Arjan W AW

Angiogenesis 20180801 3

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the eva ...[more]

PMID: 29766399

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Project description:IntroductionClinical practice guidelines (CPG) contain recommendations that aim to guide physicians in the diagnosis of and therapeutic approach toward patients affected by gastrointestinal (GI) pathologies. These CPG systematically combine scientific evidence and clinical judgment, culminating in recommendations that have been shown to improve patient care.Material and methodsEuropean and North American guidelines published in the area of gastroenterology in 2018 and 2019 were considered for inclusion. To standardize the results, only guidelines that used GRADE as an evidence system were included. Thus, in the end, 1,233 recommendations from 29 guidelines published between 2018 and 2019 were analyzed.ResultsOf the 1,233 recommendations collected, 324 (26.3%) had a low level of evidence and 127 (10.3%) had a very low level of evidence, indicating little evidence or expert opinion. Of the 29 publications analyzed, 14 (48.3%) did not present any recommendation with a high level of evidence. Regarding the 1,233 individual recommendations expressed in these 29 publications, only 336 (27.25%) assumed a high level of evidence, with 277 (82.44%) referring to liver pathology. Of the recommendations evaluated, 77 were from North American societies and the remaining 1,156 were European recommendations. In relation to the first group, only 3 (3.9%) had a high level of evidence belonging to the Guidelines for Sedation and Anesthesia in GI Endoscopy.ConclusionsMore than 25% of all recommendations currently accepted to guide patients with gastroenterological disorders are based on low-quality evidence or expert opinion. Thus, these documents should guide our performance, but clinical sense and multidisciplinarity must not be overlooked in dubious cases and with weak scientific evidence. Research should focus on the development of randomized controlled trials and systematic reviews to improve the evidence supporting the guidelines that guide clinical practice.

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Consensus guidelines for the use and interpretation of angiogenesis assays.

Publications

Consensus guidelines for the use and interpretation of angiogenesis assays.

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