Ontology highlight
ABSTRACT:
SUBMITTER: Riad A
PROVIDER: S-EPMC6238005 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Riad Aladdin A Zeng Chenbo C Weng Chi-Chang CC Winters Harrison H Xu Kuiying K Makvandi Mehran M Metz Tyler T Carlin Sean S Mach Robert H RH
Scientific reports 20181115 1
CRISPR/Cas gene studies were conducted in HeLa cells where either PGRMC1, TMEM97 or both proteins were removed via gene editing. A series of radioligand binding studies, confocal microscopy studies, and internalization of radiolabeled or fluorescently tagged LDL particles were then conducted in these cells. The results indicate that PGRMC1 knockout (KO) did not reduce the density of binding sites for the sigma-2 receptor (σ2R) radioligands, [<sup>125</sup>I]RHM-4 or [<sup>3</sup>H]DTG, but a red ...[more]