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Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system.

ABSTRACT: The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.

SUBMITTER: Ji X

PROVIDER: S-EPMC6238286 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system.

Ji Xiang X Si Xiaomin X Zhang Yi Y Zhang Huawei H Zhang Feng F Gao Caixia C

Genome biology 20181115 1

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-targe ...[more]

PMID: 30442181

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