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Accelerated FRET-PAINT microscopy.

ABSTRACT: Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.

SUBMITTER: Lee J

PROVIDER: S-EPMC6249777 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Accelerated FRET-PAINT microscopy.

Lee Jongjin J Park Sangjun S Hohng Sungchul S

Molecular brain 20181122 1

Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules sho ...[more]

PMID: 30466455

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