Dataset Information

The Contribution of Purifying Selection, Linkage, and Mutation Bias to the Negative Correlation between Gene Expression and Polymorphism Density in Yeast Populations.

ABSTRACT: The negative correlation between the rate of protein evolution and expression level of a gene has been recognized as a universal law of the evolutionary biology (Koonin 2011). In our study, we apply a population-based approach to systematically investigate the relative importance of unequal mutation rate, linkage, and selection in the origin of the expression-polymorphism anticorrelation. We analyzed the DNA sequence of protein coding genes of 24 Saccharomyces cerevisiae and 58 Schizosaccharomyces pombe strains. We found that highly expressed genes had a substantially decreased number of polymorphic sites when compared with genes transcribed less extensively. This expression-dependent reduction was especially strong in the nonsynonymous sites, although it was also present in the synonymous sites and untranslated regions, both up and down of a gene. Most importantly, no such trend was found in introns. We used these observations, as well as analyses of site frequency spectra and data from mutation accumulation experiments, to show that the purifying selection acting on nonsynonymous sites was the main, but not exclusive, factor impeding molecular evolution within the coding sequences of highly expressed genes. Linkage could not fully explain the observed pattern of polymorphism within the untranslated regions and synonymous sites, although the contribution of selection acting directly on synonymous variants was extremely small. Finally, we found that the impact of mutational bias was rather negligible.

SUBMITTER: Marek A

PROVIDER: S-EPMC6250307 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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The Contribution of Purifying Selection, Linkage, and Mutation Bias to the Negative Correlation between Gene Expression and Polymorphism Density in Yeast Populations.

Marek Agnieszka A Tomala Katarzyna K

Genome biology and evolution 20181101 11

The negative correlation between the rate of protein evolution and expression level of a gene has been recognized as a universal law of the evolutionary biology (Koonin 2011). In our study, we apply a population-based approach to systematically investigate the relative importance of unequal mutation rate, linkage, and selection in the origin of the expression-polymorphism anticorrelation. We analyzed the DNA sequence of protein coding genes of 24 Saccharomyces cerevisiae and 58 Schizosaccharomyc ...[more]

PMID: 30321329

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Project description:The trajectories of postglacial range expansions, the occurrence of lineage patches and the formation and maintenance of secondary contact between lineages may mostly reflect neutral demographic processes, including density blocking, that may leave long-lasting genetic signatures. However, a few studies have recently shown that climate may also play a role. We used red deer, a large, mobile herbivore that is assumed to be sensitive to climate change, to test hypotheses of possible selection on the mitochondrial DNA cytochrome b gene (mtDNA cytb) and competitive and/or density-blocking (using mtDNA control region). We searched for a possible link between the phylogeographic structure and abiotic climatic variables. Finally, we tested for isolation by distance and isolation by environment and assessed the impact of human-mediated translocations on the genetic structure of red deer. Our analysis of 30 red deer populations in Poland using the mtDNA control region (N = 357) and cytochrome b (N = 50) markers not only confirmed the presence of the Western and South-Eastern lineages of the species but also indicated the presence of a previously unnoticed, rare relic haplotype that grouped together C. e. italicus from Italy (the Mesola deer). No significant signs of positive selection were detected for the mtDNA cytb gene in the studied red deer. However, a significant signal for purifying selection was found in our study that may explain the narrowness of the contact zone because gene flow between the Western and South-Eastern lineages should drive relatively strong mito-nuclear incompatibilities. MtDNA control region differentiation among red deer populations in Poland correlated with different abiotic climatic variables. Strikingly, the southernmost ice sheet limits during the Elsterian was the most important factor, and it explained the largest amount of variation. However, neither isolation by distance (IBD) nor isolation by environment (IBE) were recorded, and a very limited impact of human translocations was evident. The above-mentioned results suggest that in contemporary red deer populations in Poland, the phylogeographic pattern is well preserved, and long-term processes (density and/or competitive blocking) still play a major role.

Dataset Information

The Contribution of Purifying Selection, Linkage, and Mutation Bias to the Negative Correlation between Gene Expression and Polymorphism Density in Yeast Populations.

Publications

The Contribution of Purifying Selection, Linkage, and Mutation Bias to the Negative Correlation between Gene Expression and Polymorphism Density in Yeast Populations.

Similar Datasets

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