Microfluidic bead encapsulation above 20 kHz with triggered drop formation.
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ABSTRACT:
SUBMITTER: Clark IC
PROVIDER: S-EPMC6251341 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Publications
Microfluidic bead encapsulation above 20 kHz with triggered drop formation.
Clark Iain C IC Abate Adam R AR
Lab on a chip 20181026 23
Microsphere beads are functionalized with oligonucleotides, antibodies, and other moieties to enable specific detection of analytes. Droplet microfluidics leverages this for single-molecule or -cell analysis by pairing beads and targets in water-in-oil droplets. Pairing is achieved with devices operating in the dripping regime, limiting throughput. Here, we describe a pairing method that uses beads to trigger the breakup of a jet into monodispersed droplets. We use the method to pair 10<sup>5</s ...[more]
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