Dataset Information

CaWRKY27 Negatively Regulates H₂O₂-Mediated Thermotolerance in Pepper (Capsicum annuum).

ABSTRACT: Heat stress, an important and damaging abiotic stress, regulates numerous WRKY transcription factors, but their roles in heat stress responses remain largely unexplored. Here, we show that pepper (Capsicum annuum) CaWRKY27 negatively regulates basal thermotolerance mediated by H₂O₂ signaling. CaWRKY27 expression increased during heat stress and persisted during recovery. CaWRKY27 overexpression impaired basal thermotolerance in tobacco (Nicotiana tabacum) and Arabidopsis thaliana, CaWRKY27-overexpressing plants had a lower survival rate under heat stress, accompanied by decreased expression of multiple thermotolerance-associated genes. Accordingly, silencing of CaWRKY27 increased basal thermotolerance in pepper plants. Exogenously applied H₂O₂ induced CaWRKY27 expression, and CaWRKY27 overexpression repressed the scavenging of H₂O₂ in Arabidopsis, indicating a positive feedback loop between H₂O₂ accumulation and CaWRKY27 expression. Consistent with this, CaWRKY27 expression was repressed under heat stress in the presence H₂O₂ scavengers and CaWRKY27 silencing decreased H₂O₂ accumulation in pepper leaves. These changes may result from changes in levels of reactive oxygen species (ROS)-scavenging enzymes, since the heat stress-challenged CaWRKY27-silenced pepper plants had significantly higher expression of multiple genes encoding ROS-scavenging enzymes, such as CaCAT1, CaAPX1, CaAPX2, CaCSD2, and CaSOD1. Therefore, CaWRKY27 acts as a downstream negative regulator of H₂O₂-mediated heat stress responses, preventing inappropriate responses during heat stress and recovery.

SUBMITTER: Dang F

PROVIDER: S-EPMC6252359 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

CaWRKY27 Negatively Regulates H2O2-Mediated Thermotolerance in Pepper (Capsicum annuum).

Dang Fengfeng F Lin Jinhui J Xue Baoping B Chen Yongping Y Guan Deyi D Wang Yanfeng Y He Shuilin S

Frontiers in plant science 20181119

Heat stress, an important and damaging abiotic stress, regulates numerous WRKY transcription factors, but their roles in heat stress responses remain largely unexplored. Here, we show that pepper (Capsicum annuum) CaWRKY27 negatively regulates basal thermotolerance mediated by H2O2 signaling. CaWRKY27 expression increased during heat stress and persisted during recovery. CaWRKY27 overexpression impaired basal thermotolerance in tobacco (Nicotiana tab ...[more]

PMID: 30510557

Similar Datasets

Project description:Endornaviruses include viruses that infect fungi, oomycetes, and plants. The genome of plant endornaviruses consists of linear ssRNA ranging in size from approximately 13-18 kb and lacking capsid protein and cell-to-cell movement capability. Although, plant endornaviruses have not been shown to cause detectable changes in the plant phenotype, they have been associated with alterations of the host physiology. Except for the association of cytoplasmic vesicles with infections by Vicia faba endornavirus, effects on the plant cell ultrastructure caused by endornaviruses have not been reported. Bell pepper endornavirus (BPEV) has been identified in several pepper (Capsicum spp.) species. We conducted ultrastructural analyses of cells from two near-isogenic lines of the bell pepper (C. annuum) cv. Marengo, one infected with BPEV and the other BPEV-free, and found cellular alterations associated with BPEV-infections. Some cells of plants infected with BPEV exhibited alterations of organelles and other cell components. Affected cells were located mainly in the mesophyll and phloem tissues. Altered organelles included mitochondrion, chloroplast, and nucleus. The mitochondria from BPEV-infected plants exhibited low number of cristae and electron-lucent regions. Chloroplasts contained plastoglobules and small vesicles in the surrounding cytoplasm. Translucent regions in thylakoids were observed, as well as hypertrophy of the chloroplast structure. Many membranous vesicles were observed in the stroma along the envelope. The nuclei revealed a dilation of the nuclear envelope with vesicles and perinuclear areas. The organelle changes were accompanied by membranous structure rearrangements, such as paramural bodies and multivesicular bodies. These alterations were not observed in cells from plants of the BPEV-free line. Overall, the observed ultrastructural cell alterations associated with BPEV are similar to those caused by plant viruses and viroids and suggest some degree of parasitic interaction between BPEV and the plant host.

Dataset Information

CaWRKY27 Negatively Regulates H₂O₂-Mediated Thermotolerance in Pepper (Capsicum annuum).

Publications

CaWRKY27 Negatively Regulates H<sub>2</sub>O<sub>2</sub>-Mediated Thermotolerance in Pepper (<i>Capsicum annuum</i>).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets