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Genomic Characterization of ?-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a.


ABSTRACT: Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a ?-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (?-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.

SUBMITTER: Ogura Y 

PROVIDER: S-EPMC6256406 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Genomic Characterization of β-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a.

Ogura Yoshitoshi Y   Seto Kazuko K   Morimoto Yo Y   Nakamura Keiji K   Sato Mitsuhiko P MP   Gotoh Yasuhiro Y   Itoh Takehiko T   Toyoda Atsushi A   Ohnishi Makoto M   Hayashi Tetsuya T  

Emerging infectious diseases 20181201 12


Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c.  ...[more]

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