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Anti-melanogenic and anti-oxidant activities of ethanol extract of Kummerowia striata: Kummerowia striata regulate anti-melanogenic activity through down-regulation of TRP-1, TRP-2 and MITF expression.


ABSTRACT: Kummerowia striata (K. striata) is used as a traditional medicine for inflammation-related therapy. To determine whether it has beneficial anti-melanogenic and anti-oxidant activities, we investigated the biological activities of the ethanol extract of Kummerowia striata (EKS) using a variety of in vitro and cell culture model systems. The anti-melanogenic activity was assessed in B16F10 melanoma cells in terms of melanin synthesis and in vitro tyrosinase inhibitory activity. The anti-oxidant assays were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). EKS showed strong anti-oxidant activities in DPPH and ABTS assays. The mRNA transcription levels and protein expression levels of tyrosinase, tyrosinase-related protein 1, tyrosinase-related protein 2, and microphthalmia-associated transcription factor decreased in a dose-dependent manner with EKS treatment. Additionally, EKS did not affect cell viability at different concentrations used in this study, indicating that the mechanism of action of EKS-mediated inhibition of melanin synthesis does not involve cytotoxicity. Also, we confirmed that p-coumaric acid and quercetin are important compounds for anti-melanogenesis and antioxidant properties of EKS. Collectively, our findings demonstrate for the first time that EKS possesses anti-melanogenic and anti-oxidant activities. Further evaluation and development of EKS as a functional supplement or cosmetic may be useful for skin whitening and reducing wrinkles.

SUBMITTER: Lee JY 

PROVIDER: S-EPMC6258129 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Anti-melanogenic and anti-oxidant activities of ethanol extract of <i>Kummerowia striata</i>: <i>Kummerowia striata</i> regulate anti-melanogenic activity through down-regulation of TRP-1, TRP-2 and MITF expression.

Lee Jae Yeon JY   Cho Young-Rak YR   Park Ju Hyoung JH   Ahn Eun-Kyung EK   Jeong Wonsik W   Shin Hyoung Seok HS   Kim Mi-Sun MS   Yang Seung Hwan SH   Oh Joa Sub JS  

Toxicology reports 20181103


<i>Kummerowia striata</i> (<i>K. striata</i>) is used as a traditional medicine for inflammation-related therapy. To determine whether it has beneficial anti-melanogenic and anti-oxidant activities, we investigated the biological activities of the ethanol extract of <i>Kummerowia striata</i> (EKS) using a variety of <i>in vitro</i> and cell culture model systems. The anti-melanogenic activity was assessed in B16F10 melanoma cells in terms of melanin synthesis and <i>in vitro</i> tyrosinase inhib  ...[more]

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