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Intramuscular preadipocytes impede differentiation and promote lipid deposition of muscle satellite cells in chickens.

ABSTRACT: BACKGROUND:Skeletal muscle satellite cells (MSC) are crucial for postnatal growth and regeneration of skeletal muscle. An interaction exists between MSC and intramuscular preadipocytes (IMPA). This study is the first to investigate the effects of IMPA on MSC in chickens and unveil the molecular mechanisms by transcriptome analysis. RESULTS:Primary MSC and IMPA were isolated from the pectoralis major muscle of 7-day-old chickens. After both cell types reached confluence, MSC were cultured alone or co-cultured with IMPA for 2 or 4 d. MSC treated for 2 d were subjected to RNA-seq. A total of 1653 known differentially expressed genes (DEG) were identified between co-cultured and mono-cultured MSC (|log2 FC|???1, FDR?

SUBMITTER: Guo L

PROVIDER: S-EPMC6258484 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Intramuscular preadipocytes impede differentiation and promote lipid deposition of muscle satellite cells in chickens.

Guo Liping L Cui Huanxian H Zhao Guiping G Liu Ranran R Li Qinghe Q Zheng Maiqing M Guo Yuming Y Wen Jie J Wen Jie J

BMC genomics 20181126 1

<h4>Background</h4>Skeletal muscle satellite cells (MSC) are crucial for postnatal growth and regeneration of skeletal muscle. An interaction exists between MSC and intramuscular preadipocytes (IMPA). This study is the first to investigate the effects of IMPA on MSC in chickens and unveil the molecular mechanisms by transcriptome analysis.<h4>Results</h4>Primary MSC and IMPA were isolated from the pectoralis major muscle of 7-day-old chickens. After both cell types reached confluence, MSC were c ...[more]

PMID: 30477424

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