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Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

ABSTRACT: Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.

SUBMITTER: Chong ZS

PROVIDER: S-EPMC6258485 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

Chong Zheng-Shan ZS Ohnishi Shuhei S Yusa Kosuke K Wright Gavin J GJ

Genome biology 20181126 1

Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-co ...[more]

PMID: 30477585

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