Ontology highlight
ABSTRACT:
SUBMITTER: Zhou W
PROVIDER: S-EPMC6258682 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Zhou Wenhua W Hu Li L Ying Liming L Zhao Zhen Z Chu Paul K PK Yu Xue-Feng XF
Nature communications 20181127 1
Although polymerase chain reaction (PCR) is the most widely used method for DNA amplification, the requirement of thermocycling limits its non-laboratory applications. Isothermal DNA amplification techniques are hence valuable for on-site diagnostic applications in place of traditional PCR. Here we describe a true isothermal approach for amplifying and detecting double-stranded DNA based on a CRISPR-Cas9-triggered nicking endonuclease-mediated Strand Displacement Amplification method (namely CRI ...[more]