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Deregulation of CRAD-controlled cytoskeleton initiates mucinous colorectal cancer via ?-catenin.


ABSTRACT: Epithelial integrity is maintained by the cytoskeleton and through cell adhesion. However, it is not yet known how a deregulated cytoskeleton is associated with cancer. We identified cancer-related regulator of actin dynamics (CRAD) as frequently mutated or transcriptionally downregulated in colorectal cancer. We found that CRAD stabilizes the cadherin-catenin-actin complex via capping protein inhibition. The loss of CRAD inhibits F-actin polymerization and subsequently disrupts the cadherin-catenin-actin complex, which leads to ?-catenin release and Wnt signalling hyperactivation. In mice, CRAD knockout induces epithelial cell integrity loss and Wnt signalling activation, resulting in the development of intestinal mucinous adenoma. With APC mutation, CRAD knockout initiates and accelerates mucinous and invasive adenoma development in the colorectum. These results define CRAD as a tumour suppressor, the inactivation of which deregulates the cytoskeleton and hyperactivates Wnt signalling thus initiating mucinous colorectal cancer. Our study reveals the unexpected roles of an actin cytoskeletal regulator in maintaining epithelial cell integrity and suppressing tumorigenesis.

SUBMITTER: Jung YS 

PROVIDER: S-EPMC6261439 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Deregulation of CRAD-controlled cytoskeleton initiates mucinous colorectal cancer via β-catenin.

Jung Youn-Sang YS   Wang Wenqi W   Jun Sohee S   Zhang Jie J   Srivastava Mrinal M   Kim Moon Jong MJ   Lien Esther M EM   Shang Joan J   Chen Junjie J   McCrea Pierre D PD   Zhang Songlin S   Park Jae-Il JI  

Nature cell biology 20181022 11


Epithelial integrity is maintained by the cytoskeleton and through cell adhesion. However, it is not yet known how a deregulated cytoskeleton is associated with cancer. We identified cancer-related regulator of actin dynamics (CRAD) as frequently mutated or transcriptionally downregulated in colorectal cancer. We found that CRAD stabilizes the cadherin-catenin-actin complex via capping protein inhibition. The loss of CRAD inhibits F-actin polymerization and subsequently disrupts the cadherin-cat  ...[more]

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