Unknown

Dataset Information

0

Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses.

ABSTRACT: Mice engrafted with components of a human immune system have become widely-used models for studying aspects of human immunity and disease. However, a defined methodology to objectively measure and compare the quality of the human immune response in different models is lacking. Here, by taking advantage of the highly immunogenic live-attenuated yellow fever virus vaccine YFV-17D, we provide an in-depth comparison of immune responses in human vaccinees, conventional humanized mice, and second generation humanized mice. We demonstrate that selective expansion of human myeloid and natural killer cells promotes transcriptomic responses akin to those of human vaccinees. These enhanced transcriptomic profiles correlate with the development of an antigen-specific cellular and humoral response to YFV-17D. Altogether, our approach provides a robust scoring of the quality of the human immune response in humanized mice and highlights a rational path towards developing better pre-clinical models for studying the human immune response and disease.

SUBMITTER: Douam F 

PROVIDER: S-EPMC6262001 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses.

Douam Florian F   Ziegler Carly G K CGK   Hrebikova Gabriela G   Fant Bruno B   Leach Robert R   Parsons Lance L   Wang Wei W   Gaska Jenna M JM   Winer Benjamin Y BY   Heller Brigitte B   Shalek Alex K AK   Ploss Alexander A  

Nature communications 20181128 1

Mice engrafted with components of a human immune system have become widely-used models for studying aspects of human immunity and disease. However, a defined methodology to objectively measure and compare the quality of the human immune response in different models is lacking. Here, by taking advantage of the highly immunogenic live-attenuated yellow fever virus vaccine YFV-17D, we provide an in-depth comparison of immune responses in human vaccinees, conventional humanized mice, and second gene  ...[more]

Similar Datasets

Transcriptomics Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses
2018-11-28 | GSE119751 | GEO
Transcriptomics Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 2: scRNA-seq)
2018-11-28 | GSE119750 | GEO
Transcriptomics Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 1: RNA-seq)
2018-11-28 | GSE119749 | GEO
Genomics Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses
| PRJNA490175 | ENA
Genomics Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 2: scRNA-seq)
| PRJNA490177 | ENA
Genomics Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 1: RNA-seq)
| PRJNA490176 | ENA
Unknown Modeling Human Cytomegalovirus in Humanized Mice for Vaccine Testing.
| S-EPMC7157227 | biostudies-literature
Unknown Human NK cells confer protection against HIV-1 infection in humanized mice.
| S-EPMC9753998 | biostudies-literature
Unknown Lupus Susceptibility Loci Predispose Mice to Clonal Lymphocytic Responses and Myeloid Expansion.
| S-EPMC9254690 | biostudies-literature
Unknown Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics.
| S-EPMC5573767 | biostudies-literature