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Differential gene expression analysis and SNP/InDel marker discovery in resistant wild Asparagus kiusianus and susceptible A. officinalis in response to Phomopsis asparagi infection.


ABSTRACT: This data article reports de novo transcriptome analysis of resistant wild Asparagus kiusianus and susceptible A. officinalis plants 24 and 48?h post-inoculation (24 and 48 hpi) with Phomopsis asparagi. Differential gene expression (DGE) analysis demonstrated that several genes involved in secondary metabolites and plant-pathogen interactions are up-regulated in resistant wild A. kiusianus relative to susceptible A. officinalis. The assembled contig sequences generated in this study were used to search single nucleotide polymorphism (SNP) and insertion/deletion (InDel) distribution in A. kiusianus and A. officinalis plants. SNP and InDel data developed from this transcriptome analysis will be used to generate a high-density linkage map to facilitate further development of molecular marker-assisted selection in A. officinalis.

SUBMITTER: Abdelrahman M 

PROVIDER: S-EPMC6262200 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Differential gene expression analysis and SNP/InDel marker discovery in resistant wild <i>Asparagus kiusianus</i> and susceptible <i>A. officinalis</i> in response to <i>Phomopsis asparagi</i> infection.

Abdelrahman Mostafa M   Mitoma Mai M   Ikeuchi Takao T   Mori Mitsutaka M   Murakami Kyoko K   Ozaki Yukio Y   Matsumoto Masaru M   Uragami Atsuko A   Kanno Akira A  

Data in brief 20181113


This data article reports <i>de novo</i> transcriptome analysis of resistant wild <i>Asparagus kiusianus</i> and susceptible <i>A. officinalis</i> plants 24 and 48 h post-inoculation (24 and 48 hpi) with <i>Phomopsis asparagi</i>. Differential gene expression (DGE) analysis demonstrated that several genes involved in secondary metabolites and plant-pathogen interactions are up-regulated in resistant wild <i>A. kiusianus</i> relative to susceptible <i>A. officinalis</i>. The assembled contig sequ  ...[more]

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