AMP-Activated Protein Kinase Alpha 2 Deletion Induces VSMC Phenotypic Switching and Reduces Features of Atherosclerotic Plaque Stability.

ABSTRACT: RATIONALE:AMP-activated protein kinase (AMPK) has been reported to play a protective role in atherosclerosis. However, whether AMPK?2 controls atherosclerotic plaque stability remains unknown. OBJECTIVE:The aim of this study was to evaluate the impact of AMPK?2 deletion on atherosclerotic plaque stability in advanced atherosclerosis at the brachiocephalic arteries and to elucidate the underlying mechanisms. METHODS AND RESULTS:Features of atherosclerotic plaque stability and the markers for contractile or synthetic vascular smooth muscle cell (VSMC) phenotypes were monitored in the brachiocephalic arteries from Apoe(-/-)AMPK?2(-/-) mice or VSMC-specific AMPK?2(-/-) mice in an Apoe(-/-) background (Apoe(-/-)AMPK?2(sm-/-)) fed Western diet for 10 weeks. We identified that Apoe(-/-)AMPK?2(-/-) mice and Apoe(-/-)AMPK?2(sm-/-) mice exhibited similar unstable plaque features, aggravated VSMC phenotypic switching, and significant upregulation of Kruppel-like factor 4 (KLF4) in the plaques located in the brachiocephalic arteries compared with those found in Apoe(-/-) and Apoe(-/-)AMPK?2(sm+/+) control mice. Pravastatin, an AMPK activator, suppressed VSMC phenotypic switching and alleviated features of atherosclerotic plaque instability in Apoe(-/-)AMPK?2(sm+/+) mice, but not in Apoe(-/-)AMPK?2(sm-/-) mice. VSMC isolated from AMPK?2(-/-) mice displayed a significant reduction of contractile proteins(smooth muscle actin-?, calponin, and SM-MHC [smooth muscle-mysion heavy chain]) in parallel with increased detection of synthetic proteins (vimentin and osteopontin) and KLF4, as observed in vivo. KLF4-specific siRNA abolished AMPK?2 deletion-induced VSMC phenotypic switching. Furthermore, pharmacological or genetic inhibition of nuclear factor-?B significantly decreased KLF4 upregulation in VSMC from AMPK?2(-/-) mice. Finally, we found that AMPK?2 deletion markedly promoted the binding of nuclear factor-?Bp65 to KLF4 promoter. CONCLUSIONS:This study demonstrated that AMPK?2 deletion induces VSMC phenotypic switching and promotes features of atherosclerotic plaque instability in nuclear factor-?B-KLF4-dependent manner.

SUBMITTER: Ding Y 

PROVIDER: S-EPMC6265658 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature