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Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum.


ABSTRACT: There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive regulatory effect of the transcription factor AurR1 on the aurofusarin gene cluster in Fusarium graminearum. Proteomic analyses showed that overexpression of AurR1 resulted in a significant increase of five of the eleven proteins belonging to the aurofusarin biosynthetic pathway. Further, the production of aurofusarin was increased more than threefold in the overexpression mutant compared to the wild type, reaching levels of 270 mg/L. In addition to biosynthesis of aurofusarin, several yet undescribed putative naphthoquinone/anthraquinone analogue compounds were observed in the overexpression mutant. Our results suggest that it is possible to enhance the aurofusarin production through genetic engineering.

SUBMITTER: Westphal KR 

PROVIDER: S-EPMC6266765 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Enhancing the Production of the Fungal Pigment Aurofusarin in <i>Fusarium graminearum</i>.

Westphal Klaus Ringsborg KR   Wollenberg Rasmus Dam RD   Herbst Florian-Alexander FA   Sørensen Jens Laurids JL   Sondergaard Teis Esben TE   Wimmer Reinhard R  

Toxins 20181121 11


There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive regulatory effect of the transcription factor AurR1 on the aurofusarin gene cluster in <i>Fusarium graminearum</i>. Proteomic analyses showed that overexpression of AurR1 resulted in a significant incr  ...[more]

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