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Article expression, purification, and characterization of Cu/ZnSOD from Panax ginseng.

ABSTRACT: Superoxide dismutase (SOD) has a strong antioxidant effect, but the traditional SOD extraction method is not the most efficient method of SOD amplification. In this study, we report the cloning of the Cu/ZnSOD gene from Panax ginseng into a temperature-regulated expression plasmid, pBV220. Cu/ZnSOD inclusion bodies were expressed in E. coli at a high level. Then, the inclusion bodies were purified by ion-exchange chromatography and molecular sieve chromatography. Finally, we obtained stable SOD in the bacterial broth, with a protein content of 965 mg/L and enzyme specific activity of 9389.96 U/mg. These results provide a foundation for future studies on the antioxidant mechanisms of ginseng and the development and application of ginseng Cu/ZnSOD.

SUBMITTER: Ding D

PROVIDER: S-EPMC6270835 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Article expression, purification, and characterization of Cu/ZnSOD from Panax ginseng.

Ding Dayong D Liu Shichao S Wang Kai K Huang Lihong L Zhao Jisheng J

Molecules (Basel, Switzerland) 20140616 6

Superoxide dismutase (SOD) has a strong antioxidant effect, but the traditional SOD extraction method is not the most efficient method of SOD amplification. In this study, we report the cloning of the Cu/ZnSOD gene from Panax ginseng into a temperature-regulated expression plasmid, pBV220. Cu/ZnSOD inclusion bodies were expressed in E. coli at a high level. Then, the inclusion bodies were purified by ion-exchange chromatography and molecular sieve chromatography. Finally, we obtained stable SOD ...[more]

PMID: 24936711

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