Bioconversion of rebaudioside I from rebaudioside A.
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ABSTRACT:
SUBMITTER: Prakash I
PROVIDER: S-EPMC6271207 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Bioconversion of rebaudioside I from rebaudioside A.
Prakash Indra I Bunders Cynthia C Devkota Krishna P KP Charan Romila D RD Ramirez Catherine C Snyder Tara M TM Priedemann Christopher C Markosyan Avetik A Jarrin Cyrille C Halle Robert Ter RT
Molecules (Basel, Switzerland) 20141028 11
To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I. ...[more]
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