Dataset Information

Four New Triterpenoids from Callicarpa kwangtungensis.

ABSTRACT: Four new triterpenoids which were identifed as 2?,3?,6?,19?-tetrahydroxy- oleanolic acid 28-O-?-D-glucopyranoside (1), 2-O-?-D-glucopyranosyloxy-3?,19?-di-hydroxyoleanolic acid (2), 2-O-?-D-glucopyranosyloxy-3?,19?-dihydroxyursolic acid (3), 2?,3?,6?,19?-tetrahydroxyursolic acid 28-O-?-D-glucopyranoside (4), were isolated from the aerial parts of Callicarpa kwangtungensis together with three known triterpenoids identified as 2?,3?,21?-trihydroxyursolic acid 28-O-?-D-glucopyranoside (5), 2?,3?,19?,23-tetrahydroxyoleanolic acid 28-O-?-D-glucopyranoside (6), 2?,3?,19?,23-tetrahydroxyursolic acid 28-O-?-D-glucopyranoside (7). Their structures were elucidated by the combination of mass spectrometry (MS), one and two-dimensional NMR experiments.

SUBMITTER: Zhou GP

PROVIDER: S-EPMC6272150 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Four New Triterpenoids from Callicarpa kwangtungensis.

Zhou Guo-Ping GP Yu Yan Y Yuan Ming-Ming MM Ji Tengfei T Fu Hui-Zheng HZ Zhong Rui-Jian RJ

Molecules (Basel, Switzerland) 20150519 5

Four new triterpenoids which were identifed as 2α,3β,6β,19α-tetrahydroxy- oleanolic acid 28-O-β-D-glucopyranoside (1), 2-O-β-D-glucopyranosyloxy-3α,19α-di-hydroxyoleanolic acid (2), 2-O-β-D-glucopyranosyloxy-3α,19α-dihydroxyursolic acid (3), 2α,3α,6β,19α-tetrahydroxyursolic acid 28-O-β-D-glucopyranoside (4), were isolated from the aerial parts of Callicarpa kwangtungensis together with three known triterpenoids identified as 2α,3β,21β-trihydroxyursolic acid 28-O-β-D-glucopyranoside (5), 2α,3α,19 ...[more]

PMID: 25996212

Similar Datasets

Project description:Background: Acute lung injury (ALI) is a complicated and severe lung disease, which is often characterized by acute inflammation. Poliumoside (POL), acteoside (ACT) and forsythiaside B (FTB) are phenylethanoid glycosides (PGs) with strong antioxidant, anti-inflammatory, and anti-apoptotic properties, which are extracted from Callicarpa kwangtungensis Chun (CK). The aim of this study was to investigate the protective effects of POL, ACT, and FTB against TNF-α-induced damage using an ALI cell model and explore their potential mechanisms. Methods and Results: MTT method was used to measure cell viability. Flow cytometry was used for detecting the apoptosis rate. Reactive oxygen species (ROS) activity was determined using fluorescence microscope. The expression of mRNA in apoptosis-related genes (Caspase 3, Caspase 8, and Caspase 9) were tested by qPCR. The effects of POL, ACT, FTB on the activities of nuclear factor erythroid-2 related factor 2 (Nrf2), nuclear factor kappa-B (NF-κB) and the expression of their downstream genes were assessed by western blotting and RT-PCR in A549 cells. In the current study, POL, ACT, and FTB dose-dependently attenuated TNF-α-induced IL-1β, IL-6 and IL-8 production, cell apoptosis, the expression of apoptosis-related genes (Caspase 3, Caspase 8, and Caspase 9) and ROS activity. POL, ACT, and FTB not only increased in the mRNA levels of antioxidative enzymes NADPH quinone oxidoreductase (NQO1), glutamate cysteine ligase catalytic subunit (GCLC), heme oxygenase (HO-1), but also decreased the mRNA levels of IL-1β, IL-6 and IL-8. Furthermore, they upregulated the expression of Keap1 and enhanced the activation of Nrf2, while decreased the expression of phosphor-IκBα (p-IκBα) and nuclear p65. In addition, no significant changes were observed in anti-inflammatory and antioxidant effects of POL, ACT, FTB following Nrf2 and NF-κB p65 knockdown. Conclusion: Our study revealed that POL, ACT, and FTB alleviated oxidative damage and lung inflammation of TNF-α-induced ALI cell model through regulating the Nrf2 and NF-κB pathways.

Dataset Information

Four New Triterpenoids from Callicarpa kwangtungensis.

Publications

Four New Triterpenoids from Callicarpa kwangtungensis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets