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Proteome and Peptidome of Vipera berus berus Venom.

ABSTRACT: Snake venom is a rich source of peptides and proteins with a wide range of actions. Many of the venom components are currently being tested for their usefulness in the treatment of many diseases ranging from neurological and cardiovascular to cancer. It is also important to constantly search for new proteins and peptides with properties not yet described. The venom of Vipera berus berus has hemolytic, proteolytic and cytotoxic properties, but its exact composition and the factors responsible for these properties are not known. Therefore, an attempt was made to identify proteins and peptides derived from this species venom by using high resolution two-dimensional electrophoresis and MALDI ToF/ToF mass spectrometry. A total of 11 protein classes have been identified mainly proteases but also l-amino acid oxidases, C-type lectin like proteins, cysteine-rich venom proteins and phospholipases A? and 4 peptides of molecular weight less than 1500 Da. Most of the identified proteins are responsible for the highly hemotoxic properties of the venom. Presence of venom phospholipases A? and l-amino acid oxidases cause moderate neuro-, myo- and cytotoxicity. All successfully identified peptides belong to the bradykinin-potentiating peptides family. The mass spectrometry data are available via ProteomeXchange with identifier PXD004958.

SUBMITTER: Bocian A

PROVIDER: S-EPMC6274168 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Proteome and Peptidome of Vipera berus berus Venom.

Bocian Aleksandra A Urbanik Małgorzata M Hus Konrad K Łyskowski Andrzej A Petrilla Vladimír V Andrejčáková Zuzana Z Petrillová Monika M Legath Jaroslav J

Molecules (Basel, Switzerland) 20161019 10

Snake venom is a rich source of peptides and proteins with a wide range of actions. Many of the venom components are currently being tested for their usefulness in the treatment of many diseases ranging from neurological and cardiovascular to cancer. It is also important to constantly search for new proteins and peptides with properties not yet described. The venom of <i>Vipera berus berus</i> has hemolytic, proteolytic and cytotoxic properties, but its exact composition and the factors responsi ...[more]

PMID: 27775574

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Project description:BackgroundThe variation of venom composition with geography is an important aspect of intraspecific variability in the Vipera genus, although causes of this variability remain unclear. The diversity of snake venom is important both for our understanding of venomous snake evolution and for the preparation of relevant antivenoms to treat envenomations. A geographic intraspecific variation in snake venom composition was recently reported for Vipera aspis aspis venom in France. Since 1992, cases of human envenomation after Vipera aspis aspis bites in south-east France involving unexpected neurological signs were regularly reported. The presence of genes encoding PLA(2) neurotoxins in the Vaa snake genome led us to investigate any neurological symptom associated with snake bites in other regions of France and in neighboring countries. In parallel, we used several approaches to characterize the venom PLA(2) composition of the snakes captured in the same areas.Methodology/principal findingsWe conducted an epidemiological survey of snake bites in various regions of France. In parallel, we carried out the analysis of the genes and the transcripts encoding venom PLA(2)s. We used SELDI technology to study the diversity of PLA(2) in various venom samples. Neurological signs (mainly cranial nerve disturbances) were reported after snake bites in three regions of France: Languedoc-Roussillon, Midi-Pyrénées and Provence-Alpes-Côte d'Azur. Genomes of Vipera aspis snakes from south-east France were shown to contain ammodytoxin isoforms never described in the genome of Vipera aspis from other French regions. Surprisingly, transcripts encoding venom neurotoxic PLA(2)s were found in snakes of Massif Central region. Accordingly, SELDI analysis of PLA(2) venom composition confirmed the existence of population of neurotoxic Vipera aspis snakes in the west part of the Massif Central mountains.Conclusions/significanceThe association of epidemiological studies to genetic, biochemical and immunochemical analyses of snake venoms allowed a good evaluation of the potential neurotoxicity of snake bites. A correlation was found between the expression of neurological symptoms in humans and the intensity of the cross-reaction of venoms with anti-ammodytoxin antibodies, which is correlated with the level of neurotoxin (vaspin and/or ammodytoxin) expression in the venom. The origin of the two recently identified neurotoxic snake populations is discussed according to venom PLA(2) genome and transcriptome data.

Project description:We analyzed seasonal variation in mortality rates in adult males and females of the European adder (Vipera berus), using data collected during a 13-year capture-recapture study (2005-2017) in a large population. We concurrently obtained quantitative information on the seasonal variation in the detectability and body condition of adders. Our results show strong seasonality in body condition, encounter, and capture rates of adult adders, and these patterns differ markedly between sexes and between breeding and nonbreeding females. Seasonal variation in mortality rates was however virtually nonexistent in males and moderately low in both breeding and nonbreeding females. In addition, we found no evidence for among-year differences in the seasonal mortality schedules of males and females. During periods of intensive basking, both males and pregnant females are highly visible for humans, but are not subject to strong natural mortality. This low susceptibility to predation is presumably induced by various factors, including the limitation of overt exposure to short periods of time and specific microhabitats, the dorsal coloration pattern that provides cryptic protection and possibly also an aposematic warning signal, and presumed seasonal differences in the foraging behavior and food requirements of natural predators. Our data provide some evidence that female adders, but not males, are relatively vulnerable to predation during the seasonal migrations between the hibernation and feeding habitats. Mortality in the females was not much elevated during their breeding years, but was notably highest in the spring of the ensuing nonbreeding year. After giving birth, reproductive females are extremely emaciated and have a weakened general condition. They then run the risk of dying from starvation either before, during, or after hibernation. The higher mortality after giving birth, that is sustained over a period of ca. 9 months, should be considered as an indirect and delayed survival cost of reproduction.

Project description:Female adders (Vipera berus) are "capital breeders" that exhibit delayed maturity and intermittent reproductive frequency. We studied the attainment of sexual maturity, the initiation of annual breeding in mature females, the energy and mortality costs associated with breeding, the length of the reproductive cycle and female lifetime reproductive frequency. We use longitudinal data obtained during an 18-year (2000-2017) mark-recapture study in a large population of adders in northern Belgium. A minority (15%) of the females gave birth to their first litter when they attained the actual age of 3 years, upon surpassing a minimum snout-vent-length of 38 cm. However, most females reproduced for the first time when they were 4 years or older. In mature females, breeding in a given year depends to a large extent on their body condition at the onset of the active season, indicating that a threshold level of energy reserves is necessary to start a reproductive cycle. During breeding years females stop growing, lose about one-third of their initial body mass and are very emaciated after parturition. The decrease in relative body mass was most notable initially in the largest and fattiest females. During the non-breeding years, females forage intensely to rebuild their fat reserves; their abilities to do so will strongly affect whether and when they engage in subsequent reproductive bouts. We used a multistate mark-recapture analytical method to simultaneously estimate the capture and survival rates of breeding and non-breeding females, a necessary procedure to obtain accurate estimates of survival probabilities. The analysis indicated much higher capture rates during the breeding years, but did not reveal a substantial effect of reproductive state on annual survival rates. Although some females were observed to breed in successive years, the reproductive cycle was most often biennial or triennial. However, most females (ca. 70%) that attained sexual maturity reproduced only once during their lifetime, while a minority of the females (ca. 5%) were observed to breed on 3-5 annual occasions. On average, females produced only ca. 1.3 litters during their reproductive lifetime. The short reproductive lifetime is a consequence not only of mortality directly related to the reproductive activities, but also of mortality associated with recovering from the weakened post-parturient body condition during the long intervals (1-2 years) between reproductive bouts.

Dataset Information

Proteome and Peptidome of Vipera berus berus Venom.

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Proteome and Peptidome of Vipera berus berus Venom.

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