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Functional Analysis of the Fusarielin Biosynthetic Gene Cluster.

ABSTRACT: Fusarielins are polyketides with a decalin core produced by various species of Aspergillus and Fusarium. Although the responsible gene cluster has been identified, the biosynthetic pathway remains to be elucidated. In the present study, members of the gene cluster were deleted individually in a Fusarium graminearum strain overexpressing the local transcription factor. The results suggest that a trans-acting enoyl reductase (FSL5) assists the polyketide synthase FSL1 in biosynthesis of a polyketide product, which is released by hydrolysis by a trans-acting thioesterase (FSL2). Deletion of the epimerase (FSL3) resulted in accumulation of an unstable compound, which could be the released product. A novel compound, named prefusarielin, accumulated in the deletion mutant of the cytochrome P450 monooxygenase FSL4. Unlike the known fusarielins from Fusarium, this compound does not contain oxygenized decalin rings, suggesting that FSL4 is responsible for the oxygenation.

SUBMITTER: Droce A 

PROVIDER: S-EPMC6274466 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Functional Analysis of the Fusarielin Biosynthetic Gene Cluster.

Droce Aida A   Saei Wagma W   Jørgensen Simon Hartung SH   Wimmer Reinhard R   Giese Henriette H   Wollenberg Rasmus Dam RD   Sondergaard Teis Esben TE   Sørensen Jens Laurids JL  

Molecules (Basel, Switzerland) 20161213 12

Fusarielins are polyketides with a decalin core produced by various species of <i>Aspergillus</i> and <i>Fusarium</i>. Although the responsible gene cluster has been identified, the biosynthetic pathway remains to be elucidated. In the present study, members of the gene cluster were deleted individually in a <i>Fusarium graminearum</i> strain overexpressing the local transcription factor. The results suggest that a <i>trans</i>-acting enoyl reductase (FSL5) assists the polyketide synthase FSL1 i  ...[more]

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