Dataset Information

IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion.

ABSTRACT: Retinal vein occlusion (RVO) is a common retinal vascular disease. RVO may be complicated by pronounced ischemia that often leads to severe loss of visual function. The present work aimed at studying the retinal proteome of RVO complicated by ischemia. In six Danish Landrace pigs RVO was induced with argon laser in the right eye of each animal. As four retinal veins were occluded, the RVO best corresponded to a central retinal vein occlusion (CRVO). Left control eyes received a similar laser treatment without inducing occlusion. RVO and retinal ischemia were verified by angiography. The retinas were collected 15 days after RVO for proteomic analysis. RVO resulted in a downregulation of proteins involved in visual perception, including rhodopsin, transducin alpha chain, and peripherin-2. RVO also caused a downregulation of proteins involved in neurotransmitter transport, including glutamate decarboxylase 1 (GAD1), glutamate decarboxylase 2 (GAD2), and complexins 2?4. RVO lead to increased contents of proteins involved in inflammation, including interleukin-18 (IL-18), S100A12, and annexin A1 (ANXA1). Immunohistochemistry revealed a general retinal upregulation of IL-18 and ANXA1 while S100A12 was highly abundant in retinal ganglion cells in RVO. IL-18 and S100A12 are likely to be driving forces in the inflammatory response of RVO complicated by ischemia. Our findings also suggest that RVO results in compromised neurotransmission and a downregulation of proteins involved in visual perception.

SUBMITTER: Cehofski LJ

PROVIDER: S-EPMC6274751 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion.

Cehofski Lasse Jørgensen LJ Kruse Anders A Kirkeby Svend S Alsing Alexander Nørgård AN Ellegaard Nielsen Jonas J Kojima Kentaro K Honoré Bent B Vorum Henrik H

International journal of molecular sciences 20181025 11

Retinal vein occlusion (RVO) is a common retinal vascular disease. RVO may be complicated by pronounced ischemia that often leads to severe loss of visual function. The present work aimed at studying the retinal proteome of RVO complicated by ischemia. In six Danish Landrace pigs RVO was induced with argon laser in the right eye of each animal. As four retinal veins were occluded, the RVO best corresponded to a central retinal vein occlusion (CRVO). Left control eyes received a similar laser tre ...[more]

PMID: 30366444

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Project description:PurposeTo identify risk factors associated with central retinal vein occlusion (CRVO) among a diverse group of patients throughout the United States.DesignLongitudinal cohort study.ParticipantsAll beneficiaries aged ? 55 years who were continuously enrolled in a managed care network for at least 2 years and who had ? 2 visits to an eye care provider from 2001 to 2009.MethodsInsurance billing codes were used to identify individuals with a newly diagnosed CRVO. Multivariable Cox regression was performed to determine the factors associated with CRVO development.Main outcome measuresAdjusted hazard ratios (HRs) with 95% confidence intervals (CIs) of being diagnosed with CRVO.ResultsOf the 494 165 enrollees who met the study inclusion criteria, 1302 (0.26%) were diagnosed with CRVO over 5.4 (± 1.8) years. After adjustment for known confounders, blacks had a 58% increased risk of CRVO compared with whites (HR, 1.58; 95% CI, 1.25-1.99), and women had a 25% decreased risk of CRVO compared with men (HR, 0.75; 95% CI, 0.66-0.85). A diagnosis of stroke increased the hazard of CRVO by 44% (HR, 1.44; 95% CI, 1.23-1.68), and hypercoagulable state was associated with a 145% increased CRVO risk (HR, 2.45; 95% CI, 1.40-4.28). Individuals with end-organ damage from hypertension (HTN) or diabetes mellitus (DM) had a 92% (HR, 1.92; 95% CI, 1.52-2.42) and 53% (HR, 1.53; 95% CI, 1.28-1.84) increased risk of CRVO, respectively, relative to those without these conditions.ConclusionsThis study confirms that HTN and vascular diseases are important risk factors for CRVO. We also identify black race as being associated with CRVO, which was not well appreciated previously. Furthermore, we show that compared with patients without DM, individuals with end-organ damage from DM have a heightened risk of CRVO, whereas those with uncomplicated DM are not at increased risk of CRVO. This finding may provide a potential explanation for the conflicting reports in the literature on the association between CRVO and DM. Information from analyses such as this can be used to create a risk calculator to identify possible individuals at greatest risk for CRVO.

Dataset Information

IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion.

Publications

IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion.

Similar Datasets

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