Dataset Information

New Strategies and In Vivo Monitoring Methods for Stem Cell-Based Anticancer Therapies.

ABSTRACT: Cancer is a devastating disease and the second cause of death in the developed world. Despite significant advances in recent years, such as the introduction of targeted therapies such as receptor tyrosine kinase inhibitors and immunotherapy, current approaches are insufficient to stop the advance of the disease and many cancer types remain largely intractable. In this review, we describe the latest and most revolutionary stem cell-based approaches for the treatment of cancer. We also summarize the emerging imaging modalities being applied for monitoring anticancer stem cell therapy success and discuss the implications of these novel technologies for precision medicine.

SUBMITTER: Wang P

PROVIDER: S-EPMC6276456 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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New Strategies and In Vivo Monitoring Methods for Stem Cell-Based Anticancer Therapies.

Wang Ping P Aguirre Aitor A

Stem cells international 20181115

Cancer is a devastating disease and the second cause of death in the developed world. Despite significant advances in recent years, such as the introduction of targeted therapies such as receptor tyrosine kinase inhibitors and immunotherapy, current approaches are insufficient to stop the advance of the disease and many cancer types remain largely intractable. In this review, we describe the latest and most revolutionary stem cell-based approaches for the treatment of cancer. We also summarize t ...[more]

PMID: 30581474

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Project description:To develop a clinically translatable method of cell labeling with zirconium 89 ((89)Zr) and oxine to track cells with positron emission tomography (PET) in mouse models of cell-based therapy.This study was approved by the institutional animal care committee. (89)Zr-oxine complex was synthesized in an aqueous solution. Cell labeling conditions were optimized by using EL4 mouse lymphoma cells, and labeling efficiency was examined by using dendritic cells (DCs) (n = 4), naïve (n = 3) and activated (n = 3) cytotoxic T cells (CTLs), and natural killer (NK) (n = 4), bone marrow (n = 4), and EL4 (n = 4) cells. The effect of (89)Zr labeling on cell survival, proliferation, and function were evaluated by using DCs (n = 3) and CTLs (n = 3). Labeled DCs (444-555 kBq/[5 × 10(6)] cells, n = 5) and CTLs (185 kBq/[5 × 10(6)] cells, n = 3) transferred to mice were tracked with microPET/CT. In a melanoma immunotherapy model, tumor targeting and cytotoxic function of labeled CTLs were evaluated with imaging (248.5 kBq/[7.7 × 10(6)] cells, n = 4) and by measuring the tumor size (n = 6). Two-way analysis of variance was used to compare labeling conditions, the Wilcoxon test was used to assess cell survival and proliferation, and Holm-Sidak multiple tests were used to assess tumor growth and perform biodistribution analyses.(89)Zr-oxine complex was synthesized at a mean yield of 97.3% ± 2.8 (standard deviation). It readily labeled cells at room temperature or 4°C in phosphate-buffered saline (labeling efficiency range, 13.0%-43.9%) and was stably retained (83.5% ± 1.8 retention on day 5 in DCs). Labeling did not affect the viability of DCs and CTLs when compared with nonlabeled control mice (P > .05), nor did it affect functionality. (89)Zr-oxine complex enabled extended cell tracking for 7 days. Labeled tumor-specific CTLs accumulated in the tumor (4.6% on day 7) and induced tumor regression (P < .05 on day 7).We have developed a (89)Zr-oxine complex cell tracking technique for use with PET that is applicable to a broad range of cell types and could be a valuable tool with which to evaluate various cell-based therapies.

Dataset Information

New Strategies and In Vivo Monitoring Methods for Stem Cell-Based Anticancer Therapies.

Publications

New Strategies and In Vivo Monitoring Methods for Stem Cell-Based Anticancer Therapies.

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