Ontology highlight
ABSTRACT:
SUBMITTER: Konishi Y
PROVIDER: S-EPMC6277225 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Konishi Yoshinobu Y Terai Kenta K Furuta Yasuhide Y Kiyonari Hiroshi H Abe Takaya T Ueda Yoshihiro Y Kinashi Tatsuo T Hamazaki Yoko Y Takaori-Kondo Akifumi A Matsuda Michiyuki M
iScience 20181120
Extracellular signal-regulated kinase (ERK) plays critical roles in T cell development in the thymus. Nevertheless, the dynamics of ERK activity and the role of ERK in regulating thymocyte motility remain largely unknown due to technical limitations. To visualize ERK activity in thymocytes, we here developed knockin reporter mice expressing a Förster/fluorescence resonance energy transfer (FRET)-based biosensor for ERK from the ROSA26 locus. Live imaging of thymocytes isolated from the reporter ...[more]