A Tethered Vesicle Assay for High-Throughput Quantification of Membrane Fission.

ABSTRACT: Membrane fission, which divides membrane surfaces into separate compartments, is essential to diverse cellular processes including membrane trafficking and cell division. Quantitative assays are needed to elucidate the physical mechanisms by which proteins drive membrane fission. Toward this goal, several experimental tools have been developed, including visualizing fission products using electron microscopy, measuring membrane shedding from a lipid reservoir, and observing fission of individual membrane tubes pulled from giant vesicles. However, no existing assay of membrane fission provides a quantitative, high-throughput measure of the distribution of vesicle curvatures generated by fission-driving proteins. Toward addressing this challenge, here we describe a novel approach that uses c

SUBMITTER: Snead WT 

PROVIDER: S-EPMC6279246 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature