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Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes.


ABSTRACT: The cell wall is a shape-defining structure that envelopes almost all bacteria and protects them from environmental stresses. Bacteria can be forced to grow without a cell wall under certain conditions that interfere with cell wall synthesis, but the relevance of these wall-less cells (known as L-forms) is unclear. Here, we show that several species of filamentous actinomycetes have a natural ability to generate wall-deficient cells in response to hyperosmotic stress, which we call S-cells. This wall-deficient state is transient, as S-cells are able to switch to the normal mycelial mode of growth. However, prolonged exposure of S-cells to hyperosmotic stress yields variants that are able to proliferate indefinitely without their cell wall, similarly to L-forms. We propose that formation of wall-deficient cells in actinomycetes may serve as an adaptation to osmotic stress.

SUBMITTER: Ramijan K 

PROVIDER: S-EPMC6279842 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes.

Ramijan Karina K   Ultee Eveline E   Willemse Joost J   Zhang Zheren Z   Wondergem Joeri A J JAJ   van der Meij Anne A   Heinrich Doris D   Briegel Ariane A   van Wezel Gilles P GP   Claessen Dennis D  

Nature communications 20181204 1


The cell wall is a shape-defining structure that envelopes almost all bacteria and protects them from environmental stresses. Bacteria can be forced to grow without a cell wall under certain conditions that interfere with cell wall synthesis, but the relevance of these wall-less cells (known as L-forms) is unclear. Here, we show that several species of filamentous actinomycetes have a natural ability to generate wall-deficient cells in response to hyperosmotic stress, which we call S-cells. This  ...[more]

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