Dataset Information

Besnoitia tarandi in Canadian woodland caribou - Isolation, characterization and suitability for serological tests.

ABSTRACT: In the present study, we report the first in vitro isolation of Besnoitia tarandi from North America and the second of B. tarandi at all. The parasite was isolated directly from the skin of a Canadian woodland caribou from the migratory ecotype. The animal belonged to the Leaf River Herd, in Northern Quebec, Canada. The isolate was designated Bt-CA-Quebec1. Sequencing of the 3'-end of the 18S rRNA gene, the complete sequence of the ITS1 and the 5'-end of the 5.8S rRNA gene of Bt-CA-Quebec1 revealed only minor differences to rDNA gene fragments of B. besnoiti. In contrast, the patterns for the microsatellite loci Bt-20 and Bt-21 varied substantially from those reported for B. besnoiti and B. bennetti. Surprisingly, the typing results in the loci Bt-6 and Bt-7 differed between Bt-CA-Quebec1 and results obtained for skin samples from caribou of the Canadian regions of Nunavut and the Northwest Territories reported by other investigators. This indicates that differences might exist among B. tarandi in caribou from different regions in Canada. Mice (?-interferon knockout) intraperitoneally inoculated with 1.2 × 106 or 1.5 × 106 bradyzoites mechanically released from skin tissue cysts fell ill 8, 9 or 18 days post inoculation. GKO mice inoculated with 3.0 × 104 tachyzoites isolated from the peritoneal cavity of a bradyzoites-inoculated mouse became ill earlier, i.e. 5 days post inoculation. Lung was the predilection site in all mice. Bt-CA-Quebec1 tachyzoites rapidly grew in MARC-145?cells and were used for antigen production. Comparative Western blot analyses revealed only a few differences between B. tarandi Bt-CA-Quebec1 and B. besnoiti Evora antigen when probed with sera collected from chronically infected caribou. Due to its fast growth in vitro, the Bt-CA-Quebec1 isolate may represent an interesting antigen source to establish B. tarandi-specific serological tools and to study the biology of this parasite species further.

SUBMITTER: Schares G

PROVIDER: S-EPMC6280010 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Besnoitia tarandi in Canadian woodland caribou - Isolation, characterization and suitability for serological tests.

Schares Gereon G Jutras Charles C Bärwald Andrea A Basso Walter W Maksimov Aline A Schares Susann S Tuschy Mareen M Conraths Franz J FJ Brodeur Vincent V

International journal for parasitology. Parasites and wildlife 20181127

In the present study, we report the first in vitro isolation of Besnoitia tarandi from North America and the second of B. tarandi at all. The parasite was isolated directly from the skin of a Canadian woodland caribou from the migratory ecotype. The animal belonged to the Leaf River Herd, in Northern Quebec, Canada. The isolate was designated Bt-CA-Quebec1. Sequencing of the 3'-end of the 18S rRNA gene, the complete sequence of the ITS1 and the 5'-end of the 5.8S rRNA gene o ...[more]

PMID: 30555783

Similar Datasets

Project description:Woodland caribou (Rangifer tarandus caribou) are threatened in Canada, with population and distribution declines evident in most regions of the country. Causes of declines are linked to landscape change from forest fires and human development, notably forestry oil and gas activities, which result in caribou habitat loss and affect ecosystem food webs. The Federal Species at Risk Act requires effective protection and restoration of caribou habitat, with actions to increase caribou survival. These requirements call for effective monitoring of caribou population trends to gauge success. Many woodland caribou populations are nearly impossible to count using traditional aerial survey methods, but demographic-based monitoring approaches can be used to estimate population trends based on population modeling of vital rates from marked animals. Monitoring programs have used a well-known simple population model (the Recruitment-Mortality [R/M] equation) to estimate demographic rates for woodland caribou, but have faced challenges in managing large data streams and providing transparency in the demographic estimation process. We present a stand-alone statistical software application using open-source software to permit efficient, transparent, and replicable demographic estimation for woodland caribou populations. We developed an easy-to-use, interactive web-based application for the R/M population model that uses a Bayesian estimation approach and provides the user flexibility in choice of prior distributions and other output features. We illustrate the web-application to the A la Pêche Southern Mountain (Central Group) woodland caribou population in west-central Alberta, Canada, during 1998-2017. Our estimates of population demographics are consistent with previous research on this population and highlight the utility of the application in assessing caribou population responses to species recovery actions. We provide example data, computer code, the web-based application package, and a user manual to guide installation and use. We also review underlying assumptions and challenges of population monitoring in this case study. We expect our software will contribute to efficient monitoring of woodland caribou and help in the assessment of recovery actions for this species. © 2019 The Authors. Wildlife Society Bulletin Published by Wiley Periodicals, Inc.

Project description:Microarray technology has enabled the measurement of comprehensive transcriptomic information. However, each data entry may reflect trivial individual differences among samples and also contain technical noise. Therefore, the certainty of each observed difference should be confirmed at earlier steps of the analyses, and statistical tests are frequently used for this purpose. Since a microarray measures a huge number of genes and the results are processed simultaneously, concerns regarding problems of multiplicity have been raised to the tests. To deal with these problems, several methodologies have been proposed, making the tests very conservative. Indeed, arbitrary tuning of the test threshold has also been introduced to relax the test conditions. However, the appropriateness of the multiplicity problems as well as the compensation methods has not been confirmed. The appropriateness of the compensation methods was checked by means of coincidence of the premises of the methodologies with the observed characteristics found in real data of two typical platforms of microarray analysis. Normality was observed in within-group data variations, supporting applications of parametric tests. However, genes displayed their own tendencies in the magnitude of variations, and the distributions of P-values were rather complex and varied; these characteristics are inconsistent with the premises of the compensation methodologies. Additionally, the appropriateness of the proposed multiplicities is reconsidered. When we observed differences in the transcriptome, the family-wise error rate should not be considered, since analyses at higher levels would not be influenced by a few false positives among the huge numbers of true information. Likely, concerns for a false discovery rate are not suitable for the point null hypotheses on expression levels, since the rate of true null hypotheses should be rare in contradiction to the premise of the methodology. Although compensation methods have been recommended to deal with the problem of multiplicity, the compensations are actually inappropriate for many of the applications of transcriptome analyses. Compensations are not only unnecessary, but will increase the occurrence of false negative errors, and arbitrary adjustment of the threshold damages the objectivity of the tests. Rather, the results of parametric tests should be evaluated directly. This SuperSeries is composed of the SubSeries listed below.

Dataset Information

Besnoitia tarandi in Canadian woodland caribou - Isolation, characterization and suitability for serological tests.

Publications

<i>Besnoitia tarandi</i> in Canadian woodland caribou - Isolation, characterization and suitability for serological tests.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets