Multiomic Strategies Reveal Diversity and Important Functional Aspects of Human Gut Microbiome.

ABSTRACT: It is well accepted that dysbiosis of microbiota is associated with disease; however, the biological mechanisms that promote susceptibility or resilience to disease remain elusive. One of the major limitations of previous microbiome studies has been the lack of complementary metatranscriptomic (functional) data to complement the interpretation of metagenomics (bacterial abundance). The purpose of this study was twofold, first to evaluate the bacterial diversity and differential gene expression of gut microbiota using complementary shotgun metagenomics (MG) and metatranscriptomics (MT) from same fecal sample. Second, to compare sequence data using different Illumina platforms and with different sequencing parameters as new sequencers are introduced, and to determine if the data are comparab

SUBMITTER: Ranjan R 

PROVIDER: S-EPMC6280293 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature