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Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy.


ABSTRACT: BACKGROUND:Increasing evidence indicates a pathogenic role of deregulated autophagy in rheumatoid arthritis (RA). We examined the relationship between autophagy and inflammatory parameters in patients with RA receiving biologic therapy. METHODS:In 72 patients with RA and 20 healthy control subjects (HC), autophagosome levels were determined by the mean fluorescence intensity (MFI) of autophagosomotropic dye incorporated into circulating immune cells, and p62 expression levels in immune cells were measured by flow cytometry. We used immunoblotting to examine protein expression of LC3-II and p62 in peripheral blood mononuclear cells. RESULTS:Patients with RA had significantly higher levels of autophagosome reflected by MFI of Cyto-ID in circulating lymphocytes, monocytes, and granulocytes (median values, 3.6, 11.6, and 64.8, respectively) compared with HC (1.9, 6.0, and 35.8; respectively) (all p?

SUBMITTER: Chen YM 

PROVIDER: S-EPMC6280483 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy.

Chen Yi-Ming YM   Chang Chun-Yu CY   Chen Hsin-Hua HH   Hsieh Chia-Wei CW   Tang Kuo-Tung KT   Yang Meng-Chun MC   Lan Joung-Liang JL   Chen Der-Yuan DY  

Arthritis research & therapy 20181205 1


<h4>Background</h4>Increasing evidence indicates a pathogenic role of deregulated autophagy in rheumatoid arthritis (RA). We examined the relationship between autophagy and inflammatory parameters in patients with RA receiving biologic therapy.<h4>Methods</h4>In 72 patients with RA and 20 healthy control subjects (HC), autophagosome levels were determined by the mean fluorescence intensity (MFI) of autophagosomotropic dye incorporated into circulating immune cells, and p62 expression levels in i  ...[more]

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