Bacillus subtilis exhibits MnmC-like tRNA modification activities.

ABSTRACT: The MnmE-MnmG complex of Escherichia coli uses either ammonium or glycine as a substrate to incorporate the 5-aminomethyl or 5-carboxymethylaminomethyl group into the wobble uridine of certain tRNAs. Both modifications can be converted into a 5-methylaminomethyl group by the independent oxidoreductase and methyltransferase activities of MnmC, which respectively reside in the MnmC(o) and MnmC(m) domains of this bifunctional enzyme. MnmE and MnmG, but not MnmC, are evolutionarily conserved. Bacillus subtilis lacks genes encoding MnmC(o) and/or MnmC(m) homologs. The glycine pathway has been considered predominant in this typical gram-positive species because only the 5-carboxymethylaminomethyl group has been detected in tRNA^Lys_UUU and bulk tRNA to date. Here, we show tha

SUBMITTER: Moukadiri I 

PROVIDER: S-EPMC6284559 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature