Charge variant native mass spectrometry benefits mass precision and dynamic range of monoclonal antibody intact mass analysis.

ABSTRACT: The preponderance and diversity of charge variants in therapeutic monoclonal antibodies has implications for antibody efficacy and degradation. Understanding the extent and impact of minor antibody variants is of great interest, and it is also a critical regulatory requirement. Traditionally, a combination of approaches is used to characterize antibody charge heterogeneity, including ion exchange chromatography and independent mass spectrometric variant site mapping after proteolytic digestion. Here, we describe charge variant native mass spectrometry (CVMS), an integrated native ion exchange mass spectrometry-based charge variant analytical approach that delivers detailed molecular information in a single, semi-automated analysis. We utilized pure volatile salt mobile phases over a pH gra

SUBMITTER: Bailey AO 

PROVIDER: S-EPMC6284562 | biostudies-literature | 2018 Nov-Dec

REPOSITORIES: biostudies-literature