Ontology highlight
ABSTRACT:
SUBMITTER: Jinek M
PROVIDER: S-EPMC6286148 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Jinek Martin M Chylinski Krzysztof K Fonfara Ines I Hauer Michael M Doudna Jennifer A JA Charpentier Emmanuelle E
Science (New York, N.Y.) 20120628 6096
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in ...[more]