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Diurnal protein oscillation profiles in Drosophila head.


ABSTRACT: Circadian clocks control daily rhythms in physiology, metabolism and behaviour in most organisms. Proteome-wide analysis of protein oscillations is still lacking in Drosophila. In this study, the total protein and phosphorylated protein in Drosophila heads in a 24-h daily time-course were assayed by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and 10 and 7 oscillating proteins as well as 19 and 22 oscillating phosphoproteins in the w1118 control and ClkJrk mutant strains were separately identified. Lastly, we performed a mini screen to investigate the functions of some oscillating proteins in circadian locomotion rhythms. This study provides the first proteomic profiling of diurnally oscillating proteins in fly heads, thereby providing a basis for further mechanistic studies of these proteins in circadian rhythm.

SUBMITTER: Du J 

PROVIDER: S-EPMC6287739 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Diurnal protein oscillation profiles in Drosophila head.

Du Juan J   Zhang Yifan Y   Xue Yongbo Y   Zhao Xiaoyun X   Zhao Xianguo X   Wei Yu Y   Li Zhen Z   Zhang Yong Y   Zhao Zhangwu Z  

FEBS letters 20181030 22


Circadian clocks control daily rhythms in physiology, metabolism and behaviour in most organisms. Proteome-wide analysis of protein oscillations is still lacking in Drosophila. In this study, the total protein and phosphorylated protein in Drosophila heads in a 24-h daily time-course were assayed by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and 10 and 7 oscillating proteins as well as 19 and 22 oscillating phosphoproteins in the w<sup>1118</sup> control and C  ...[more]

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