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A transcriptomics analysis of the Tbx5 paralogues in zebrafish.

ABSTRACT: TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels.

SUBMITTER: Boyle Anderson EAT

PROVIDER: S-EPMC6287840 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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A transcriptomics analysis of the Tbx5 paralogues in zebrafish.

Boyle Anderson Erin A T EAT Ho Robert K RK

PloS one 20181210 12

TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues res ...[more]

PMID: 30532148

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Tbx5 paralogues during zebrafish development

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Tbx5 paralogues during zebrafish development

Project description:Tbx5 paralogues during zebrafish development

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Essential roles of zebrafish rtn4/Nogo paralogues in embryonic development.

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Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos.

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