Ontology highlight
ABSTRACT:
SUBMITTER: Cowman SA
PROVIDER: S-EPMC6289444 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Cowman Steven A SA James Phillip P Wilson Robert R Cookson William O C WOC Moffatt Miriam F MF Loebinger Michael R MR
PloS one 20181211 12
The diagnosis of pulmonary non-tuberculous mycobacterial disease (pNTM) is dependent on the isolation of NTM in culture, which is prone to overgrowth and contamination and may not capture the diversity of mycobacteria present, including rare or unidentified species. This study aimed to develop a culture independent method of detecting and identifying mycobacteria from sputum samples using partial sequencing of the hsp65 gene. DNA was extracted from sputum samples from subjects with pNTM and dise ...[more]