Unknown

Dataset Information

0

CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages.


ABSTRACT: Regulatory mechanisms controlling the pool size of spleen dendritic cells (DC) remain incompletely understood. DCs are continuously replenished from hematopoietic stem cells, and FLT3-mediated signals cell-intrinsically regulate homeostatic expansion of spleen DCs. Here we show that combining FLT3 and CSF1R-deficiencies results in specific and complete abrogation of spleen DCs in vivo. Spatiotemporally controlled CSF1R depletion reveals a cell-extrinsic and non-hematopoietic mechanism for DC pool size regulation. Lack of CSF1R-mediated signals impedes the differentiation of spleen macrophages of embryonic origin, and the resulted macrophage depletion during development or in adult mice results in loss of DCs. Moreover, embryo-derived macrophages are important for the physiologic regeneration of DC after activation-induced depletion in situ. In summary, we show that the differentiation of DC and their regeneration relies on ontogenetically distinct spleen macrophages, thereby providing a novel regulatory principle that may also be important for the differentiation of other hematopoietic cell types.

SUBMITTER: Percin GI 

PROVIDER: S-EPMC6290072 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages.

Percin Gulce Itir GI   Eitler Jiri J   Kranz Andrea A   Fu Jun J   Pollard Jeffrey W JW   Naumann Ronald R   Waskow Claudia C  

Nature communications 20181211 1


Regulatory mechanisms controlling the pool size of spleen dendritic cells (DC) remain incompletely understood. DCs are continuously replenished from hematopoietic stem cells, and FLT3-mediated signals cell-intrinsically regulate homeostatic expansion of spleen DCs. Here we show that combining FLT3 and CSF1R-deficiencies results in specific and complete abrogation of spleen DCs in vivo. Spatiotemporally controlled CSF1R depletion reveals a cell-extrinsic and non-hematopoietic mechanism for DC poo  ...[more]

Similar Datasets

| S-EPMC9316889 | biostudies-literature
2013-04-10 | E-GEOD-41417 | biostudies-arrayexpress
| S-EPMC8185375 | biostudies-literature
| S-EPMC5659290 | biostudies-literature
2013-04-10 | GSE41417 | GEO
| S-EPMC10835270 | biostudies-literature
| S-EPMC3853406 | biostudies-literature
| S-EPMC4545768 | biostudies-literature
| S-EPMC5066309 | biostudies-literature
| S-EPMC5413334 | biostudies-literature