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Exploring the influence from whole blood DNA extraction methods on Infinium 450K DNA methylation.

ABSTRACT: Genome-wide DNA methylation studies are becoming increasingly important in unraveling the epigenetic basis of cell biology, aging and human conditions. The aim of the present study was to explore whether different methods for extracting DNA from whole blood can affect DNA methylation outcome, potentially confounding DNA methylation studies. DNA was isolated from healthy blood donors (n = 10) using three different extraction methods (i.e. two automatic extractions methods based on magnetic beads or isopropanol precipitation, and manual organic extraction). DNA methylation was analyzed using the Infinium HumanMethylation450 Bead Chip (Infinium 450K) (n = 30 samples in total), which is a frequently used method in genome-wide DNA methylation analyses. Overall, the different extraction methods did not have a significant impact on the global DNA methylation patterns. However, DNA methylation differences between organic extraction and each of the automated methods were in general larger than differences between the two automated extraction methods. No CpG sites or regions reached genome-wide significance when testing for differential methylation between extraction methods. Although this study is based on a small sample, these results suggest that extraction method is unlikely to confound Infinium 450K methylation analysis in whole blood.

SUBMITTER: Hjorthaug HS

PROVIDER: S-EPMC6291135 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Exploring the influence from whole blood DNA extraction methods on Infinium 450K DNA methylation.

Hjorthaug Hanne Sagsveen HS Gervin Kristina K Mowinckel Petter P Munthe-Kaas Monica Cheng MC

PloS one 20181212 12

Genome-wide DNA methylation studies are becoming increasingly important in unraveling the epigenetic basis of cell biology, aging and human conditions. The aim of the present study was to explore whether different methods for extracting DNA from whole blood can affect DNA methylation outcome, potentially confounding DNA methylation studies. DNA was isolated from healthy blood donors (n = 10) using three different extraction methods (i.e. two automatic extractions methods based on magnetic beads ...[more]

PMID: 30540848

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Exploring the influence from whole blood DNA extraction methods on Infinium 450K DNA methylation.

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Exploring the influence from whole blood DNA extraction methods on Infinium 450K DNA methylation.

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