Epitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domains.

ABSTRACT: OBJECTIVE:To identify the specific domains of the presynaptic protein synapsin targeted by recently described autoantibodies to synapsin. METHODS:Sera of 20 and CSF of two patients with different psychiatric and neurological disorders previously tested positive for immunoglobulin (Ig)G antibodies to full-length synapsin were screened for IgG against synapsin I domains using HEK293 cells transfected with constructs encoding different domains of rat synapsin Ia. Additionally, IgG subclasses were determined using full-length synapsin Ia. Serum and CSF from one patient were also screened for IgA autoantibodies to synapsin I domains. Sera from nine and CSF from two healthy subjects were analyzed as controls. RESULTS:IgG in serum from 12 of 20 IgG synapsin full-length positive patients, but from

SUBMITTER: Mertens R 

PROVIDER: S-EPMC6292584 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature