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Essential role of ultraviolet radiation in the decrease of corneal endothelial cell density caused by pterygium.

ABSTRACT: PURPOSE:To investigate the essential role of ultraviolet (UV) radiation in the decrease of corneal endothelial cell density (ECD) caused by pterygium. METHODS:In this retrospective controlled study, 76 consecutive patients with unilateral primary pterygium were enrolled from January 2016 to August 2016. The Central corneal ECD was measured in both eyes. Divide the subjects into two subgroups according to the UV exposure time, and the difference of corneal ECD between both eyes was analyzed using independent samples t-test. The relationship between the percentage of difference of corneal ECD and the disease duration, the percentage of pterygium to the cornea and the astigmatism value were analyzed. RESULTS:For subjects with longer UV exposure, the average of ECD in pterygium group and control group were, respectively, 2264.83?±?349.78 cells/mm2 and 2477.16?±?300.36 cells/mm2 (P?=?0.004). For subjects with short UV exposure, the average of ECD in pterygium group and control group were, respectively, 2596.46?±?373.24 cells/mm2 and 2690.66?±?321.34 cells/mm2 (P?=?0.262). The decrease of corneal ECD was positively correlated with the period of the disease (P?=?0.035). There was no statistical correlation between a decrease in the corneal ECD and the percentage of pterygium to cornea (R?=?-0.055, P?=?0.635) and the astigmatism value in the pterygium eye (R?=?-0.135, P?=?0.246). CONCLUSIONS:UV radiation exposure played a significant role in the effect of pterygium decreasing the corneal ECD.

SUBMITTER: Li X

PROVIDER: S-EPMC6292900 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Essential role of ultraviolet radiation in the decrease of corneal endothelial cell density caused by pterygium.

Li Xia X Dai Yiqin Y Xu Weiwei W Xu Jianjiang J

Eye (London, England) 20180829 12

<h4>Purpose</h4>To investigate the essential role of ultraviolet (UV) radiation in the decrease of corneal endothelial cell density (ECD) caused by pterygium.<h4>Methods</h4>In this retrospective controlled study, 76 consecutive patients with unilateral primary pterygium were enrolled from January 2016 to August 2016. The Central corneal ECD was measured in both eyes. Divide the subjects into two subgroups according to the UV exposure time, and the difference of corneal ECD between both eyes was ...[more]

PMID: 30158575

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Project description:PurposeCorneal endothelial cell density (ECD) gradually decreases after corneal transplantation by unknown biologic, biophysical, or immunologic mechanism. Our purpose was to assess the association between donor corneal endothelial cell (CEC) maturity in culture and postoperative endothelial cell loss (ECL) after successful corneal transplantation.DesignProspective cohort study.ParticipantsThis cohort study was conducted at Baptist Eye Institute, Kyoto, Japan, between October 2014 and October 2016. It included 68 patients with a 36-month follow-up period who had undergone successful Descemet stripping automated endothelial keratoplasty (DSAEK) or penetrating keratoplasty.MethodsHuman CECs (HCECs) from remaining peripheral donor corneas were cultured and evaluated for maturity by surface markers (CD166+, CD44-/dull, CD24-, and CD105-) using fluorescence-activated cell sorting. Postoperative ECD was assessed according to the mature-differentiated HCEC contents: high-maturity group: > 70%, middle-maturity group: 10% to 70%, low-maturity group: < 10%. The successful rate of ECD maintained at 1500 cells/mm2 at 36 months postoperative was analyzed using the log-rank test.Main outcome measuresEndothelial cell density and ECL at 36 months postoperative.ResultsThe 68 included patients (mean [standard deviation] age 68.1 [13.6] years, 47.1% women, 52.9% DSAEK). The high, middle, and low-maturity groups included 17, 32, and 19 eyes, respectively. At 36 months postoperative, the mean (standard deviation) ECD significantly decreased to 911 (388) cells/mm2 by 66% in the low-maturity group, compared with 1604 (436) by 40% and 1424 (613) cells/mm2 by 50% in the high and middle-maturity groups (P < 0.001 and P = 0.007, respectively) and the low-maturity group significantly failed to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001). Additional ECD analysis for patients who underwent DSAEK alone displayed a significant failure to maintain ECD at 1500 cells/mm2 at 36 months postoperative (P < 0.001).ConclusionsThe high content of mature-differentiated HCECs expressed in culture by the donor peripheral cornea was coincident with low ECL, suggesting that a high-maturity CEC content predicts long-term graft survival. Understanding the molecular mechanism for maintaining HCEC maturity could elucidate the mechanism of ECL after corneal transplantation and aid in developing effective interventions.Financial disclosuresProprietary or commercial disclosure may be found after the references.

Project description:Purpose To investigate the effects of pterygium on corneal cell and nerve density in patients with unilateral pterygium using in vivo laser scanning confocal microscopy (LSCM). Methods In this cross-sectional study, 24 patients with unilateral pterygium who were treated in the Department of Ophthalmology of the Second People's Hospital of Wuxi City from April 2018 to July 2018 were analyzed. Each eye with pterygium and its fellow eye were imaged by LSCM. The density of basal corneal epithelial cells, anterior stromal cells, posterior stromal cells, dendritic cells, and endothelial cells in pterygium and adjacent clear cornea was measured. In the fellow eyes, the central cornea, nasal cornea, nasal mid-peripheral cornea, and temporal cornea were imaged. The difference in the density of cells and subepithelial nerve fibers in different corneal regions of eyes with pterygium was analyzed. The cell and nerve density of the fellow cornea were also measured to exclude the influencing factors. Results The density of corneal basal epithelial cells in the central corneas of eyes with pterygium was 6497?±?1776 cells/mm2, which was higher than that in the area near the head of pterygium (5580?±?1294 cells/mm2, P < 0.001), the region above pterygium (6097?±?1281 cells/mm2, P=0.049), and the region below pterygium (5463?±?1007 cells/mm2, P=0.001). The density of anterior stromal cells in the central cornea was 742?±?243 cells/mm2, which was higher than that in the area near the head of pterygium (587?±?189 cells/mm2, P=0.005), the region below pterygium (492?±?159 cells/mm2, P=0.005), and the temporal cornea (574?±?164 cells/mm2, P=0.003). The density of endothelial cells in the central cornea was 2398?±?260 cells/mm2, which was higher than that in the area near the head of pterygium (2296?±?231 cells/mm2, P=0.011) and the region below pterygium (2272?±?400 cells/mm2, P=0.020). The density of dendritic cells in the central cornea was 53?±?48 cells/mm2, which was lower than that in the area near the head of pterygium (250?±?224 cells/mm2, P=0.001), the upper region (103?±?47 cells/mm2, P=0.006), and the lower region (90?±?48 cells/mm2, P=0.023). The corneal nerve fiber length (CNFL) in the center was higher than that in the area near the head of pterygium, the upper region, and the lower region. Compared with fellow eyes, eyes with pterygium had a significantly higher mean corneal power (KM) (P < 0.001). There was a significant positive linear relationship between the corneal area invaded by pterygium of pterygia and KM (r?=?0.609, P=0.009). Conclusion Basal epithelial cells, stromal cells, endothelial cells, dendritic cells, and subepithelial nerve fibers in the central cornea of eyes with pterygium were different from those of pterygium and adjacent clear cornea. LSCM is effective for observing the morphology and quantity of corneal cells in pterygium.

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Essential role of ultraviolet radiation in the decrease of corneal endothelial cell density caused by pterygium.

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Essential role of ultraviolet radiation in the decrease of corneal endothelial cell density caused by pterygium.

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